HPTLC method was developed and validated for estimation of tramadol HCl in human plasma by drug spiking method. Chromatographic separation was achieved on silicagel 60F254 aluminium plates (10 x 10 cm) as stationary phase, using mobile phase comprising toluene: 2-propanol: ammonia (8:2:0.1 v/v/v) with detection at 271 nm. The method was linear (r2>0.998) in the concentration range of 200-1000 ng/spot in human plasma. The % CV for interday and intraday precisions was 1.48-2.09% and 1.11-1.65%, respectively. The limit of quantification and the limit of detection were 75.08 ng/spot and 24.78 ng/spot, respectively.
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